Zoic acid (DTNB) which is then read at 412 nm. Determination of lipid peroxidation product (malondialdehyde, MDA) in brain tissue homogenate This method is based on the determination of malondialdehyde (MDA), an end product of lipid peroxidation, which reacts with thiobarbituric acid to yield a pink coloured trimethine complex exhibiting a maximum absorbance at 530-532 nm [28]. HPLC determination
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Ded many of the proteins that had been found to be decreased under baseline conditions in uninfected SP-A-/- mice as compared to WT mice. Our working hypotheses to explain these differences are: a) that expression of these proteins is stimulated by the induction of infection, probably as a consequence of increased levels of regulatory molecules such as cytokines; b) in order to compensate for the
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E been identified by MALDI-ToF/ToF are circled and numbered and all proteins are named in Table 1. Note that in cases where multiple isoforms are circled, the identity of all isoforms has been confirmed by MALDI-ToF/ToF.below those seen in the corresponding infected WT mice, resulting in a regulatory pattern that is almost the inverse of that described above for most of the proteins. These protein
E been identified by MALDI-ToF/ToF are circled and numbered and all proteins are named in Table 1. Note that in cases where multiple isoforms are circled, the identity of all isoforms has been confirmed by MALDI-ToF/ToF.below those seen in the corresponding infected WT mice, resulting in a regulatory pattern that is almost the inverse of that described above for most of the proteins. These protein
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